Coagulase-negative staphylococci in ewe udder surface and raw milk samples

Coagulase-negative staphylococci (CNS) are among the major causes of subclinical mastitis in dairy ewe flocks. This has a financially significant impact on sector and consumer health. The determination bacterial count, particularly CNS, is critical terms quantity quality ovine milk. Thus, purpose this study was to quantify CNS count udder surface raw milk samples ewe, addition identifying strains (n=8) collected from individual by 16S rRNA gene sequencing. A total 164 originated four sheep farms were tested. obtained values compared between different farms. Furthermore, during 2018 2019 case Farm I compared. Significantly higher (p<0.05) average observed taken (2.8±1.0 lg CFU/cm2) than that III (2.3±0.6 CFU/cm2). In milk, value derived (3.5±0.9 CFU/mL), while significantly lower IV (1.8±0.4 CFU/mL). bulk tank samples, highest mean 5.3±0.4 CFU/mL, there no (p>0.05) variation counts decreased certain extent both sample types tested except for Tsigai breed. correlation very weak (r=0.048). Staphylococcus simulans, auricularis, equorum identified molecular sequencing simulans most frequently species. indicates possible contamination milking storage. Therefore, further studies required investigate other sources improve hygienic